Nanostructured Immunosensors. Application to the detection of Progesterone

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dc.contributor.author Pingarrón J.M. en_US
dc.contributor.author Yáñez-Sedeño P. en_US
dc.contributor.author González-Cortés A. en_US
dc.contributor.author Carralero V. en_US
dc.date.accessioned 2007-02-20T13:15:29Z
dc.date.available 2007-02-20T13:15:29Z
dc.date.issued 2006 en_US
dc.identifier.citation European Nano Systems 2006, Paris, France, 14-15 December 2006, ENS 2006, Co-locating with Nano Transfer 2006, The 2nd Worshop on Nano Technology Transfer in Europe, p. 100-103 en_US
dc.identifier.isbn 2-916187-05-7 en_US
dc.identifier.uri http://hdl.handle.net/2042/6794
dc.description.abstract Nanostructured electrodes. Immunosensor. ProgesteroneA novel nanostructured electrochemical immunsensor for the determination of progesterone is reported. The approach combines the properties of gold nanoparticles with the use of a graphite-Teflon composite electrode matrix, into which gold nanoparticles are incorporated by simple physical inclusion. The antibody anti-progesterone was directly attached to the electrode surface. The immunosensor functioning is based on competitive assay between progesterone and alkaline phosphatase-labelled progesterone. Monitoring of the affinity reaction was accomplished by the electrochemical oxidation of 1-naphtol. Modification of the graphite -Teflon electrode matrix with gold nanoparticles improves substantially the electrooxidation response of 1-naphtol. Using a detection potential of +0.3V, a detection limit for progesterone of 0.84 ng ml-1 was obtained. Analysis of seven milk samples spiked at a 3.5 ng ml-1 progesterone concentration level yielded a mean recovery of 101+6%. Detection of the antigen-antibody reaction with a graphite - Teflon - colloidal - gold - Tyrosinase electrode, using phenylphosphate as alkaline phosphatase substrate to generate phenol, which is subsequently reduced at -0.1 V at the composite electrode, produced a high improvement in the sensitivity for progesterone detection en_US
dc.format.extent 136986 bytes
dc.format.mimetype application/pdf
dc.language.iso EN en_US
dc.publisher TIMA Editions , Grenoble, France en_US
dc.rights http://irevues.inist.fr/utilisation en_US
dc.source European Nano Systems 2006, Paris, France, 14-15 December 2006, ENS 2006, Co-locating with Nano Transfer 2006, The 2nd Worshop on Nano Technology Transfer in Europe, p. 100-103 en_US
dc.subject Nanostructured electrodes. Immunosensor. Progesterone en_US
dc.title Nanostructured Immunosensors. Application to the detection of Progesterone en_US
dc.type Conference proceeding en_US
dc.contributor.affiliation University Complutense of Madrid [Spain] en_US
dc.contributor.affiliation University Complutense of Madrid [Spain] en_US
dc.contributor.affiliation University Complutense of Madrid [Spain] en_US
dc.contributor.affiliation University Complutense of Madrid [Spain] en_US


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