Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy

Show full item record

Files in this item

PDF 1022.pdf 794.7Kb

Pour citer ce document :
URI: http://hdl.handle.net/2042/6271
Title: Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy
Author: Lin, Ching-Ting; Tien, En-Kuang; Lee, Szu-Yuan; Lu, Long-Sheng; Wu, Chau-Chung; Dong, Chen-Yuan; Lin, Chii-Wann
Abstract: Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.
Subject: Two-photon microscopy, atherosclerosis, ox-LDL, autofluorescence
Publisher: TIMA Editions , Grenoble, France
Date: 2006

This item appears in the following Collection(s)

Show full item record





Advanced Search